相关科研杂志审稿人:Plos One, FCT, JAFC, JSFA, Food Contron, IUBMB Life, J Biotechnol,、Anal Biochem, 农业生物技术学报,生物工程学报,食品科学,东北农业大学学报等
长期从事“生物源风险因子检测与毒理评价”领域的研究工作,在生物毒素检测及致毒机制,转基因食品检测与安全评价, 生物源掺伪检测技术,食品微生物检测技术4个生物源风险因子研究领域开展研究工作长期从事“生物源风险因子检测与毒理评价”领域的研究工作,在生物毒素检测及致毒机制,转基因食品检测与安全评价, 生物源掺伪检测技术,食品微生物检测技术4个生物源风险因子研究领域开展研究工作
在食品安全领域已经发表已发表论文100余篇,其中SCI收录60余篇,教育部成果鉴定7项,申请专利20余项,获得授权国家专利16余项。
主编和参编教材10部包括《转基因食品安全评价与检测技术》(主编),《食品安全案例解析》(主编),《转基因食品社会文化伦理透视》(主编),《食品营养与安全》(主编),《转基因食品》(主编),《国际转基因生物食用安全检测及其标准化》(副主编),《食品安全检测技术》(参编),和《食品生物技术实验指导》(参编)等。
部分代表性论文(通讯作者*):
40 Ochratoxin A induced early hepatotoxicity: new mechanistic insights from microRNA, mRNA and proteomic profiling studies. Scientific reports. 2014,4: 5163. X.Z Qi, X. Yang, S.Y. Chen, X.Y. He, H. Dweep, M.Z. Guo, W.H. Cheng, W.T. Xu*, Y.B. Luo, N. Gretz, Q. Dai, K.L. Huang
39 DNA damage and S phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK 293). Mutat Res-Fund Mol M. 2014,765: 22-31. Q. Yang, X.Y. He, X.H. Li, W.T. Xu*, Y.B. Luo, X. Yang, Y. Wang, Y.C. Li, K.L. Huang
38 microRNA profilingof rats with ochratoxin A nephrotoxicity. BMC Genomics. 2014,15:333. Q. Dai, J. Zhao, X.Z. Qi, W.T. Xu*, X.Y He, M.Z. Guo, H. Dweep, W.H. Cheng, Y.B. Luo, K. Xia, N. Gretz, K.L. Huang
37 Protective effect of N-acetylcysteine against DNA damage and S-phase arrest induced by Ochratoxin A in human embryonic kidney cells (HEK-293). Food Chem. Toxicol. 2014,70:40-47. Q. Yang, L. Shi, K.L. Huang, W.T. Xu*
36 Development and Application of a Quantitative Loop mediated Isothermal Amplification Method for Detecting Genetically Modified Maize MON863. J. Sci. Food Agric. 2014,DOI: 10.1002/jsfa.6707. S.C. Huang, Y.C Xu, X.H. Yan, Y. Shang, P.Y. Zhu , W.Y. Tian , W.T. Xu*.
35 Central role of Nix in the autophagic response to orchratoxin A Food Chem. Toxicol. 2014,69:202-209. X.L. Shen, B.Y. Zhang, R. Liang, W.H. Cheng, W.T. Xu*, Y.B. Luo, C.H. Zhao, K.L. Huang.
34 Analysis of Individual and Combined Effects of Ochratoxin A and Zearalenone on HepG2 and KK-1 Cells with Mathematical Models. Toxins. 2014,6(4):1177-92. Y.Z. Li, B.Y. Zhang, X.Y. He, W.H. Cheng, W.T. Xu*, Y.B. Luo, R. Liang , H.S. Luo, K.L. Huang.
33 Protective role of the mitochondrial Lon protease 1 in ochratoxin A-induced cytotoxicity in HEK293 cells. Journal of Proteomics. 2014,101:154-68. B.Y. Zhang, X.L. Shen, R. Liang, Y.Z. Li, K.L. Huang, C.H. Zhao, Y.B. Luo, W.T. Xu*.
32 Restriction enzyme cutting site distribution regularity for DNA looping technology
Gene. 2014,534(2):222-228. Y. Shang, N. Zhang, P.Y. Zhu, Y.B. Luo, K.L. Huang, W.Y. Tian, W.T. Xu*.
31 A peach (Prunuspersica)-specific gene, Lhcb2, used as an endogenous reference gene for qualitative and real-time quantitative PCR to detect fruit products. LWT-Food Science and Technology. 2014,55(1):218-223 Y Shang, P Zhu, K Huang, W Liu, W Tian, Y Luo, W.T. Xu*.
30 Randomly broken fragment PCR with 5’ end-directed adaptor for genome walking. Scientific reports. 2013, 3: 3465. W.T. Xu, Y. Shang, P.Y. Zhu, Z.F. Zhai, J.He, K.L. Huang, Y.L. Luo.
29 An iTRAQ-based mitoproteomics approach for profiling the nephrotoxicity mechanisms of ochratoxin A in HEK 293 cells. Journal of Proteomics. 2013,78(14):398-415. X.L. Shen, Y. Zhang, W.T. Xu*, R. Liang, J.J. Zheng, Y.B. Luo, Y. Wang, K.L. Huang
28 Zinc protects HepG2 cells against the oxidative damage and DNA damage induced by ochratoxin A. Toxicol Appl Pharm. 2013,268(2):123-131. J Zheng, Y Zhang, W.T. Xu*, YB Luo, J Hao, X Shen, X Yang, X Li, K Huang.
27 Comparative proteomics and physiological characterization of Arabidopsis thaliana seedlings in responses to Ochratoxin A. Plant molecular biology. 2013,82(4-5):321-337. Y Wang, J Hao, W Zhao, Z Yang, W Wu, Y Zhang, W.T. Xu*, YB Luo, K Huang.
26 A 90-day feeding study of glyphosate-tolerant maize with the G2-aroA gene in Sprague–Dawley rats. Food Chem. Toxicol. 2013,51: 280-287. Y Zhu, X He, Y, S Zou,X Zhou, KHuang, W Xu*.
25 Effects of genetically modified T2A-1 rice on the GI health of rats after 90-day supplement. Scientific reports. 2013,3:1962. Y Yuan, W Xu*, X He, H Liu, S Cao, X Qi, K Huang, Y Luo.
24 The transcriptional response of virulence genes in Listeria monocytogenes during inactivation by nisin. Food Control. 2013,31(2):519–524. H Shi,Q Trinh, W Xu*, Y Luo, W Tian, K Huang.
23 Development and optimization of an efficient method to detect the authenticity of edible oils. Food Control. 2013,31(1):71-79. J He, W Xu*, Y Shang, P Zhu, X Mei, W Tian, K Huang.
22 Single universal primer multiplex ligation-dependent probe amplification with sequencing gel electrophoresis analysis. Analytical Biochemistry. 2013,443(2):243-248. Y Shang, P Zhu, W Xu*, T Guo, W Tian, Y Luo, K Huang.
21 Restriction enzyme cutting site distribution regularity for DNA looping technology. Gene. 2013,534(2):222-8.Y Shang, N Zhang, P Zhu, Y Luo, K Huang, W Tian, W Xu*
20 Transcript and protein profiling analysis of OTA-induced cell death reveals the regulation of the toxicity response process in Arabidopsis thaliana. Journal of Experimental Botany. 2012,63(5):2171-2087. Y Wang, X Peng, W Xu*, Y Luo, W Zhao, J Hao, Z Liang, Y Zhang, K Huang.
19 A novel Universal Primer-Multiplex-PCR method with sequencing gel electrophoresis analysis. PLOS ONE. 2012,7(1): e22900. W Xu, Z Zhai, K Huang, N Zhang, Y Yuan, Y Shang, Y Luo.
18 A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast. PLOS ONE. 2012,7(3): e32943. S Cao, W Xu*, N Zhang, Y Wang, Y Luo, X He, K Huang.
17 Safety assessment of transgenic Bacillus thuringiensis rice T1c-19 in Sprague–Dawley rats from metabonomics and bacterial profile perspectives. IUBMB Life. 2012, 64(3):242-250. S Cao, X He, W Xu*, Y Luo,Y Yuan, P Li, B Cao, H Shi, K Huang
16 The effect of genetically modified Lactobacillus plantarum 590 on the gut health of Sprague–Dawley rats. IUBMB Life. 2012,64(7):617–627. H Liu, W Xu*, Y Yuan, S Cao, X He, S Li, K Huang, Y Luo.
15 Loop-linker PCR: An advanced PCR technique for genome walking. IUBMB Life. 2012,64(10):841–845. Q Trinh, H Shi, W Xu*, J Hao, Y Luo, K Huang.
14 A universal primer multiplex PCR method for typing of toxinogenic Pseudomonas aeruginosa. Appl. Microbiol. Biot. 2012,95(6):1579-87. H Shi, Q Trinh, W Xu*, B Zhai, Y Luo, K Huang.
13 Subchronic feeding study of stacked trait genetically-modified soybean (3Ø5423×40-3-2) in Sprague-Dawley rats. Food Chem. Toxicol. 2012,50(9): 3256-3263. X Qi, X He, Y Luo, S Li, S Zou, S Cao, M Tang, B Delaney, W Xu*, K Huang.
12 Safety assessment of dehydration-responsive element-binding (DREB) 4 protein expressed in E. coli. Food Chem. Toxicol. 2012,50(11): 4077-4084. B Cao, X He, Y Luo, L Ma, P Liu, S Cao, X Liu,S Zou, W Xu*,K Huang
11 A 90-day subchronic feeding study of genetically modified maize expressing Cry1Ac-M protein in Sprague–Dawley rats. Food Chem. Toxicol. 2012,50(9): 3215–3221. P Liu, X He, D Chen, Y Luo, S Cao, H Song, T Liu, K Huang, W Xu*.
10 An A-T Linker Adapter PCR Method for Chromosome Walking without Restriction-site Cloning Bias. Analytical Biochemistry. 2012,425: 62–67. Q Trinh, W Xu* , H Shi, Y Luo, K Huang.
9 Establishment of a viable cell detection system for microorganisms in wine based on ethidiummonoazide and quantitative PCR. Food Control. 2012, 27(1): 81-86. H Shi, W Xu*, Q Trinh, Y Luo, Z Liang, Y Li, K Huang.
8 Potential allergenicity research of Cry1C protein from genetically modified rice. Regul Toxicol Pharm. 2012,63: 181–187. S Cao; X He; W Xu*, Y Luo; W Ran; L Liang; Y Dai; K Huang.
7 Comparative analysis of the proteomic and nutritional composition of transgenic rice seeds with Cry1ab/ac genes and their non-transgenic counterparts. Journal of cereal science. 2012,55(2):236-233. Y Wang, W Xu*, W Zhao, J Hao, Y Luo, X Tang, Y Zhang, K Huang.
6 Establishment and optimization of a wheat germ cell-free protein synthesis system and its application in venom kallikrein. Protein Expres. Purif. 2012,84(2):173-80. Y Wang, W Xu*, X Kou, Y Luo, Y Zhang, B Ma, M Wang, K Huang.
5 Metabonomics Study of Transgenic Bacillus Thuringiensis Rice (T2A-1) Meal in a 90-Day Dietary Toxicity Study in Rats. Molecular BioSystems. 2011,7:2304 -2310. S Cao, W Xu*, Y Luo, X He, Y Yuan, W Ran, L Liang, K Huang.
4 The effect of various environmental factors on the ethidium monazite and quantitative PCR method to detect viable bacteria. J Appl. Microbiol. 2011,111, 1194–1204. H Shi., W Xu*, Y Luo., L Chen, Z Liang, X Zhou, K Huang.
3 Safety assessment of Cry1C protein from genetically modified rice according to the national standards of PR China for a new food resource. Regul Toxicol. Pharm. 2010,58,474–481. S Cao, X He, W Xu*, W Ran, L Liang, Y Luo, Y Yuan, N Zhang, X Zhou, K Huang.
2 Event-specific Detection of Stacked Genetically Modified Maize Bt11×GA21 by UP-M-PCR and Real-time PCR. J. Agric. Food Chem. 2009,57: 395–402. W Xu, Y Yuan, L Luo, W Bai, C Zhang, K Huang.
1 Safety Assessment of Cry1Ab/Ac fusion protein. Food Chem. Toxicol. 2009,47:1459–1465. W Xu, S Cao, X He, Y Luo, X Guo, Y Yuan, K Huang.
1、参与和主持完成国家级课题20余项,主要有国家自然基金“转基因产品品系特异性检测新技术的研究”(主持人)、教育部青年教师科研项目“线粒体参与调控拟南芥抗赭曲霉毒素A毒性作用机制的初步研究”(主持)、转基因专项子任务“转基因作物精准检测相关研究”(主持人)、转基因专项子任务“转基因作物代谢产物检测相关研究”(主持人)、转基因专项子任务“转基因作物食用安全关键技术相关研究”(主持人)、国家标准“转基因生物及其产品食用安全检测 蛋白氨基酸序列检测方法” (主持人)、转基因专项子任务“转基因生物食用安全能力建设相关课题”(主持人)、国家“863”项目“复合性状转基因农产品精准检测技术研究”(2006AA10Z440)(副主持)、农业部项目“转基因农作物检测与标识追溯体系的研究” (副主持)、农业部项目“农产品市场转基因成分例行监测” (副主持)、农业部“948”项目“外源基因删除技术的引进及其在我国转基因作物研发中的应用”(2008-Z41)(主要参加人)、“农业转基因生物及其产品安全检测技术、关键设备及相关材料的引进”(引进国际先进农业科学技术项目 948项目 2005-Z32)(主要参加人)、北京市重点项目“粮油原料中主要真菌毒素检测及控制技术研究及应用示范”(子课题主持)、农业部“948”项目“国际农业转基因生物安全评价、检测技术体系与标准化体系的引进与建立”(2007-Z8)(主要参加人)、国家“十五”重大专项“食品安全关键技术”子课题“食品包装、贮藏与运输过程中的安全控制技术”(主要参加人员)等;
2、目前参与和主持的在课题10余项,主要有国家“863计划”“食用农产品中病原菌安全控制技术”(任务主持), 国家科技支撑计划“产毒真菌核酸快速检测技术的研究” (子任务主持),转基因重大专项"复合性状转基因成分检测、溯源技术及标识阈值研究"(课题主持),“转基因作物核酸精准检测技术研究-转基因作物快速检测及品系鉴定新技术研究” (任务主持),“新性状转基因植物安全评价与控制技术及转基因产品定量标识阈值研究”(任务主持),国家科技支撑计划“食源性致病菌高通量现场快速检测关键技术及其设备的研发”(任务副主持),教育部青年教师科研项目“microRNA组学研究赭曲霉毒素A毒性在体内体外模型中的联系与差异"(主持),北京市科技新星项目“食源性致病菌新型等温PCR现场快速检测及核酸试纸条关键技术研究”(主持),教育部新世纪人才项目“ASK1参与调控赭曲霉毒素A诱导的肾细胞毒性作用机制研究”(主持)。
主讲2门本科生课程以及2门研究生实验课程,参讲6门课程,转基因食品社会文化伦理透视(主讲), 食品安全案例热点透视(主讲)16学时、 、双向凝胶电泳(2-DE)实验技术(主讲)8学时、酶联免疫(ELISA)实验(主讲)8学时;指导本科生URP,毕业设计.